PriMera Scientific Medicine and Public Health (ISSN: 2833-5627)

Research Article

Volume 5 Issue 4

Combined effect of AlCl3 and GA on Biochemical Characterization of Wheat Primary Leaves during Dark Incubated Senescence

G Fareeda* and SDS Murthy

October 04, 2024

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Abstract

Senescence is the age-dependent end of the life span. In plants, it can be visualized by yellowing of leaves that accompanies the mobilization of leaf nutrients to the reproductive structures. The yellowing of senescing leaves is correlated with a series of biochemical changes such as loss of chlorophyll contents, degradation of proteins, RNA and a decline in photosynthetic activity. Senescence limits crop yields in annual crops like maize, rice and wheat. Delayed leaf senescence is a desirable agronomic trait to improve crop yield. In this study the combination of AlCl3 and Gibberellic acid reduced the loss of pigments, proteins, spectral properties, electron transport activities in wheat primary leaves under incubated dark conditions. The restoration of whole chain electron transport activity by the combination of AlCl3 and GA was closely associated with the restoration of PS II activity when compared with PS I. The combination of Gibberellic acid and AlCl3 treated leaf thylakoid membranes showed an increase in absorption at 680 nm moderate increases at 480 nm and 440 nm at 72 h during dark incubation. The AlCl3 and Gibberellic acid protected the degradation of water oxidation complex polypeptides (33, 23, 17 KDa) of PS II and slightly protected the 68 kDa of PS I polypeptides.

Keywords: Senescence retardation; PSII and PS I activity; water oxidation complex polypeptides

References

  1. Arnon DI. “Copper enzymes in isolated chloroplasts”. Poly phenol oxidase in Beta vulgaris. Plant Physiol 24 (1949): 1-15.
  2. Doorn WG., et al. “Delay of Iris flower senescence by cytokinins and jasmonates”. Physiol Plant 148.1 (2013): 105-120.
  3. Geeta Chandra Reddy KS and Mohan Ram HY. “Extension of vase life of cut marigold and Chrysanthemum flowers by the use of cobalt chrolide”. Indian J. Exp. Biol 19 (1981): 150-154.
  4. Janečková H., et al. “The interplay between cytokinins and light during senescence in detached Arabidopsis leaves”. Plant Cell Environ 41.8 (2018): 1870-1885.
  5. Krieger-Liszkay A. “Generation of reactive oxygen species in thylakoids from senescing flag leaves of the barley varieties Lomerit and Carina”. Planta 241.6 (2015): 1497-508.
  6. Krieger-Liszkay A, Krupinska K and Shimakawa G. “The impact of photosynthesis on initiation of leaf senescence”. Physiol. Plant 166 (2019): 148-164.
  7. Laemmli UK. “Cleavage of structural proteins during the assembly of the head of bacteriophage T4”. Nature 227 (1970): 680-685.
  8. Li F, Chung T and Vierstra RD. “Autophagy-related II plays a critical role in general autophagy- and senescence-induced mitophagy in Arabidopsis”. Plant Cell 26 (2014): 788-807.
  9. López-Otín C., et al. “The hallmarks of aging”. Cell 153 (2013): 1194-1217.
  10. Lowry OH., et al. “Protein measurement with the poly phenol reagent”. J. Biol. Chem 193 (1951): 265-275.
  11. Maillard A., et al. “Leaf mineral nutrient remobilization during leaf senescence and modulation by nutrient deficiency”. Front. Plant Sci 6 (2015): 317.
  12. Pedhadiya MD, Vaishnav PP and Singh YD. “Development of photosynthetic electron transport reactions under the influence of phytohormones and nitrate nutrition in greening cucumber cotyledons”. Photosynth Res 13 (1987): 159-165.
  13. Saha S and Good NE. “Products of the photo phosphorelation reactions”. J. Biol. Chem 245 (1970): 5017-5021.
  14. Smart CM., et al. “The timing of maize leaf senescence and characterisation of senescence related cDNAs”. Physiol. Plant 93 (1995): 673-682.
  15. Subhan D and SDS Murthy. “Senescence Retarding Effect of Metal Ions: Pigment and Protein Contents and Photochemical Activities of Detached Primary Leaves of Wheat”. Photosynthetica 39.1 (2001): 53-58.
  16. Swamy PM, Murthy SDS and Suguna P. “Retardation of dark induced invitro alterations in PS II organization of cowpea leaf discs by combination of Ca2+ and benzyladenine”. Boil. Plant 37 (1995): 457-460.
  17. Wu X., et al. “Effects of cytokinin on photosynthetic gas exchange, chlorophyll fluorescence parameters and antioxidative system in seedlings of eggplant (Solanum melongena L.) under salinity stress”. Acta Physiologiae Plantarum 34 (2012): 2105-2114.
  18. Yuan L and Xu DQ. “Stimulation effect of gibberellic acid short-term treatment on leaf photosynthesis related to the increase in Rubisco content in broad bean and soybean”. Photosynth. Res 68 (2001): 39-47.
  19. Zhang H, Mishra D and Samal B. “Interaction of benzimidazole and nickel in delaying senescence of detached rice leaves”. Z. naturforsch 26c (2013): 1377.
  20. Zhang K., et al. “Salicylic acid 3-hydroxylase regulates Arabidopsis leaf longevity by mediating salicylic acid catabolism”. Proc Natl Acad Sci USA 110 (2013): 14807-14812.