PriMera Scientific Surgical Research and Practice (ISSN: 2836-0028)

Research Article

Volume 6 Issue 5

Haemophilus influenzae in North Africa: Two Decades of Vaccine Impact, Serotype Shifts, and Antimicrobial Resistance

Mzilem Sabrine*

October 27, 2025

Abstract

Background: Haemophilus influenzae (Hi) remains a significant pathogen, causing invasive and non-invasive infections worldwide. Before Hib conjugate vaccines, serotype b (Hib) was the leading cause of meningitis, pneumonia, and septicemia in children under five, with high morbidity and mortality, particularly in Africa. In North Africa, Hib vaccination programs introduced in the early 2000s drastically reduced invasive Hib disease. However, this success has been followed by two key shifts: the rise of non-typeable H. influenzae (NTHi) as the predominant cause of invasive disease and increasing antimicrobial resistance (AMR), particularly to β-lactam antibiotics.

Materials and Methods: This review synthesizes two decades of data from Tunisia, Morocco, Algeria, Egypt, and Libya on vaccine impact, serotype dynamics, and evolving resistance. Published studies, surveillance reports, and immunization data were analyzed, including the prevalence of different serotypes and resistance patterns to antibiotics. Molecular techniques for detecting β-lactamase production and penicillin-binding protein (PBP) mutations were also reviewed.

Results: Hib incidence dropped significantly post-vaccination, with declines of 75-93% in Morocco and near elimination in Tunisia. However, NTHi and serotype a emerged as leading invasive strains. AMR to ampicillin rose from ~27% to >40%, linked to β-lactamase production and PBP3 mutations. Despite this, third-generation cephalosporins remain effective, though some regions show reduced susceptibility.

Conclusion: Hib vaccines have been successful, but the rise of NTHi and AMR presents new challenges. Sustained vaccine coverage, molecular surveillance, and novel vaccines targeting NTHi are critical.

References

  1. World Health Organization. “Global burden of Haemophilus influenzae type b disease”. WHO (2000).
  2. Mzilem S and Boukhchina S. “Typeable Ampicillin-resistant Haemophilus influenzae Strains in Tunisian Children”. Medicine (Baltimore) 101.38 (2022): e30713.
  3. Ikken Y., et al. “Epidemiology of bacterial meningitis in Morocco (2015-2018)”. Acta Microbiol Immunol Hung 67 (2020): 243-251.
  4. Ikken Y., et al. “Real-time PCR detection of H. influenzae from culture-negative CSF samples in Morocco”. Acta Microbiol Immunol Hung 68 (2021): 107-112.
  5. Moroccan Ministry of Health. “Hib vaccination impact report, 2007-2009”. Rabat, Morocco.
  6. Mzilem S and Boukhchina S. “Resistance to several antibiotics in Haemophilus influenzae strains”. Acta Med Mediterranea 37.1 (2021): 421-424.
  7. Pre-vaccine surveillance, Tunisia 1999-2002. National Institute of Public Health, Tunisia.
  8. Benbachir M., et al. “Antimicrobial resistance of respiratory pathogens in North Africa, 2006-2007”. J Chemother 21 (2009): 56-60.
  9. Peltola H. “Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis”. Clin Microbiol Rev 13 (2000): 302-317.
  10. World Health Organization. “Expanded Programme on Immunization guidelines”. WHO (2012).
  11. Libya Health Ministry Reports, unpublished data, 2000-2023.
  12. El-Mokhtar MA., et al. “Community-acquired pneumonia caused by H. influenzae in adults, Egypt 2013-2014”. Sci-Afric J Sci Issues Res Essays 2 (2014): 456-461.