PriMera Scientific Medicine and Public Health (ISSN: 2833-5627)

Review Article

Volume 5 Issue 2

Effect of Gongronema latifilium and Luffa aegyptiaca decoction on the blood glucose, lipids and liver enzymes of alloxan induced diabetic rats

Ngozi Mercy Nnam, Ngozi Helen Onyia and Ijioma John Okorie*

July 29, 2024

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Abstract

There is lack of information on the use of Gongronema latifilium and Luffa aegyptiaca leafy vegetables decoction in the management of diabetes. The study investigated the effect of G. latifilium and L. aegyptiaca leafy vegetable decoction on blood glucose level, lipid profile and liver enzymes of alloxan-induced diabetic wistar rats. Experimental design was adopted to randomly assign 30 rats of 5 treatment groups and 1 untreated group each. The leafy vegetables were purchased, picked separately and washed with deionized water. The vegetables were separately boiled for 10.3minutes in 3.5L and 4.5L of water, respectively. The decoctions were sieved out separately and stored in the refrigerator for further study. Alloxan was used to induce diabetes in the rats, while treatment lasted for 21days. Phytochemicals, acute toxicity test, blood glucose and liver function test were done using standard methods. Pair-sample t-test (p < 0.05) was used to determine the effect of the decoctions and percentage difference was calculated. There were 69.64% and 68.88% decreases in the blood glucose level of diabetic rats treated with 3mL/kg bodyweight of G. latifilium and L. aegyptiaca decoctions, respectively. There was significant increase (p < 0.05) in the HDL-C level of diabetic rats after treatment with 3mL/kg bodyweight of G. latifilium and L. aegyptiaca decoctions. Significant decrease (p < 0.05) was observed in the liver enzymes activity of the diabetic rats after treatment with 3mL/kg bodyweight of G. latifilium and L. aegyptiaca decoctions. The leafy vegetables decoction had potentials to reduce high blood glucose level, dyslipidemia and, for normal liver function.

Keywords: Gongronema latifilium; Luffa aegyptiaca; decoction; blood glucose; diabetic rats; liver enzymes; dyslipidemia

References

  1. Dhanush Krishna BJ, Suguna RY and Satyanarayana MU. “Serum insulin levels and lipid profiles of streptozotocin induced diabetic wistar rats”. J. Ind. Vet. Assoc., Kerala 10.2 (2014): 26.
  2. World Health Organization. Traditional Medicine, Fact sheet. NA 134 (2001).
  3. Nnam NM, Onyechi UA and Madukwe E. “Nutrients and phytochemical composition of some leafy vegetables with medicinal significance Nig”. J. of Nutr. Sci 33.2 (2012): 15-19.
  4. Fifa TV., et al. “In vivo biological effects of two antidiabetic medicinal plants used in Benin as folk Medicine”. J Clinc. Proteomics (2013).
  5. Elizues RC., et al. “Use of traditional medicine intreatment of diabetesin eastern Uganda, A qualitative exploration for reasons for choice”. Journal of International Health and Human Right 3 (2013): 145-147.
  6. Harborne JB. “Phytochemical methods: a guide to modern techniques of plant analysis”. Chapman and Hill, London, (1973): 182-201.
  7. Boham BA and Kocopal -Abiyazan R. Flavonoid and condensed tannin from the leaves of hawiian vaccinium vaticulatium and V. calycinium Parcific science (1994).
  8. Olivera CS. Nutraceutical phytochemicals and antioxidants, Extension fact sheet HYG.5050 (2003).
  9. Lorke D. “A new approach to practical acute toxicity testing”. Arch. Toxicol 53 (1984): 275-289.
  10. Lott JA and Turner K. Evaluation of trunders glucose oxidase method for measuring the blood glucose, AHM Viswanatheis (1975).
  11. Albers JJ, Warmick GR and Cheng MC. “Determination of high density lipoprotein (HDL)-cholesterol Lipid”. Journal of Lipid Biochemistry 13 (1978): 926-932.
  12. Assman GN., et al. Determination of low density lipoprotein cholesterol Clinical Chemical Acts 140 (1984): 77-83.
  13. Trinder P. “Determination of blood glucose using an oxidase peroxide system with non-carcinogenic chromogen”. Journal of Clinical and Chemical Pathology 22 (1969): 158-161.
  14. Allian CE., et al. “Enzymatic determin ation of serum Cholesterol”. J of Clin Chem 20 (2012): 470-475.
  15. Reitman S and Frankel S. “Colorimetric Point Method”. American J. Pathology 28 (1957): 56-63.
  16. Bamisaye FO., et al. “Evaluation of hypoglycemic and toxicological effects of extracts of Morinda lucida in hyperglycemic albino rats”. International Research Journal of Biochemistry and Bioinformatics 3.2 (2013): 37-43.
  17. Akroum S. “Analytical and Biological Study of Natural Flavonoids”. Ph.D thesis. pp. 113, Mentouri University of Constantine, Algeria (2011).
  18. Ugochukwu HN and Babady NE. Antioxidant effects of Gongronema latifolium in hepatocytes of rat models of non-insulin dependent diabetes mellitus (2002).
  19. Kenjiro TD., et al. “Inhibition of alpha glucosidase and alpha amylase by flavonoids”. J. Nutr. Sci 52.2 (2006): 149-153.
  20. Alyemeni ME, Basahy AG and Sher HP. “Physico-chemical analysis and mineral composition of some sesame seeds (Sesamum indicum L.) grown in the Gizan area of Saudi Arabia”. J. Med. Plants Res 5.2 (2011): 270-274.
  21. Morel ST. “Phytochemical and biological evaluation of Derrisferruginea Benth”. (Fabaceae). University of Angers, Int. J. Curr. Microbiol. App. Sci 2.8 (2011): 1-13.
  22. Hanhineva KZ, Torronen RP and Bonica-pons II. “Impact of dietary polyphenol on carbohydrate metabolism”. Int.J Moi Sci 11.4 (2010): 1365-1402.
  23. Otamere HO., et al. “Lipid profile in diabetes mellitus; what impact has age and duration”. British Journal of Pharmacology and Toxicology 2.3 (2011): 135-137.
  24. Inbaraj SD and Inbaraj GJ. “Comparative study of lipid and glycemic effects of pioglitazone, rosiglitazone with glibenclamide in patients with type 2 Diabetes and Dyslipidemia”. Global Journal of Pharmacology 8.1 (2014) 107-113.
  25. Ugochukwu SC, Arukwe UI and Onuoha I. “Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker”. Asian Journal of Plant Science and Research 3.3 (2013): m 10-13.
  26. Edet EN., et al. “Effect of Gongronema latifolium Leaf Extract on some Liver Enzymes and Protein Levels in Diabetic and non-Diabetic Rats”. J. of Pharm. and med (2012).
  27. Rajkapoor B., et al. “Protective effect of Phyllanthus polyphyllus on acetaminophen induced hepatotoxicity in rats”. Pakistan Journal of Pharmaceutical Sciences 21 (2008): 57-62.