PriMera Scientific Medicine and Public Health (ISSN: 2833-5627)

Research Article

Volume 3 Issue 2

Antibiotic Resistance Phenotypes of Escherichia Coli Strains Isolated from Urinary Tract Infections in the Medical Biology Laboratory of Niamakoro FOMBA Hospital in Segou, Mali

Diarra L*, Guindo I, Kalambry AC, Coulibaly K, Thiero M, Koné A, Keita-Traore M and Ouedraogo AS

July 22, 2023

DOI : 10.56831/PSMPH-03-079


Introduction: In order to guide antibiotic therapy, most often probabilistic this study aimed to characterize the different phenotypes of antibiotic resistance of E. coli strains responsible for urinary tract infections. Materials and methods: This was a prospective cross-sectional study with descriptive purposes that took place from January to December 2020 at the medical biology laboratory of Niamakoro FOMBA Hospital in Ségou. Uriselect 4 Agar was used for urine culture followed by biochemical identification by API 20E gallery. The antibiogram was then performed by the disc method according to the 2019 recommendations of the antibiogram committee of the French Society of Microbiology. Results: During this study, 56 enterobacteriaceae were isolated and identified in urinary tract infections, including 43 strains of E. coli or 76.79%. Regarding resistance patterns, the ESBL phenotype was the most observed with 46.51% for the beta-lactam class. Aminoglycoside resistance affected 41.46% of strains resistant to all aminoglycosides tested. Quinolone resistance was identified with 88.10% of strains resistant to all quinolones. ESBL profiles were associated with resistance to aminoglycosides, fluoroquinolones and sulfamethoxazole + trimethoprim in 13.95% of cases. Conclusion: The present study on resistance phenotypes in E. coli isolates reports worrisome results for the main classes of antibiotics.

Keywords: Antibiotics; E. coli; urinary tract infection; resistance phenotypes


  1. World Health Organisation. Antimicrobial resistance: Global Report on Surveillance 2014. Geneva: WHO (2014).
  2. Hassaine H and Boulanoir M. “Antibiotic resistance of Escherichia coli isolated from urinary tract infections at Tizi Ouzou hospital”. Akli Mohand Oulhadj-Bouira University, Morocco (2019): 74.
  3. Ouedraogo AS., et al. “Emergence and spread of antibiotic resistance in West Africa: enabling factors and threat assessment”. Tropical Health Medicine 27 (2017): 147-154.
  4. Toudji AG., et al. “Prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae strains isolated in Togo and their susceptibility to antibiotics”. International Journal of Biological Chemical Sciences 11.3 (2017): 1165-1177.
  5. Kalambry AC., et al. “Beta-lactam resistance profile of enterobacteriaceae isolated from urine samples at the Mali Hospital”. Malian Journal of Infectiology and Microbiology 14 (2019): 6-13.
  6. Rakotovao-Ravahatra ZD., et al. “Phenotypes of resistance of Escherichia coli strains responsible for urinary tract infection in the laboratory of the University Hospital Center of Befelatanana Antananarivo”. Pan Afr Med J 26 (2017): 166.
  7. French Society of Microbiology Enterobacteriaceae. In: CASFM / EUCAST: French Society of Microbiology Ed (2019): 1-45.
  8. Romli A., et al. “ESBL Enterobacteriaceae for urinary tract infections: epidemiology and resistance”. MOROCCO MEDICAL 33.1 (2011): 12-17.
  9. Hailaji NS, Ould Salem ML and Ghaber SM. “Antibiotic susceptibility of uropathogenic bacteria in the city of Nouakchott – Mauritania”. Prog Urol 26.6 (2016): 346-52.
  10. Sissoko T. “Urinary tract infections in Bamako: epidemiological and bacteriological aspects”. University of Bamako, Mali (2006): 103.
  11. Sbiti M, Lahmadi K and Louzi L. “Epidemiological profile of uropathogenic enterobacteriaceae producing extended-spectrum beta-lactamases”. Pan African Medical Journal 28.29 (2017): 1-8.
  12. El Bouamri MC., et al. “Current antibiotic resistance profile of uropathogenic Escherichia coli strains and therapeutic consequences”. Advances in Urology 16 (2014): 1058-62.
  13. Elhani D. “Extended-spectrum beta-lactamases: the challenge is increasing”. Ann Biol Clin 70.2 (2012): 117-40.
  14. Gonsu Kamga H., et al. “Resistance phenotypes of Escherichia coli strains responsible for community-acquired urinary tract infections in the city of Yaoundé, Cameroon”. African Journal of Pathology and Microbiology 3 (2014): 1-4.
  15. Nguyen JC and Lambert T. “Phenotypic interpretation of antibiogram vis-à-vis aminoglycosides”. Francophone Journals Laboratories 445 (2012): 75-7.
  16. Batard E., et al. “From antibiotic consumption to bacterial resistance: the example of Escherichia coli resistance to quinolones”. Therapeutic medicine 17.4 (2011): 294-301.
  17. Tagajdid MR., et al. “Study of the resistance of Escherichia coli strains isolated in urine to fluoroquinolones and third-generation cephalosporins”. Medicine and infectious diseases 40.2 (2010): 70-3.