Incidence, Antibiotic Susceptibility Pattern and Plasmid Profile of Bacterial Pathogens from Surgical site Infections in a Tertiary Hospital in Lagos, Nigeria
Dauphin Dighitoghi Moro*, Hakeem Olanrewaju Bello, Taiwo Oluwabukola Bello, Ukhureigbe Miriam Oluchi, Hammed Kolawole Shittu, Zenas Chisom Agubata, Chinyere Theresa Nzomiwu, Collins Chidiebere Nwaji and Jude Chidozie Nwaji
June 28, 2023
DOI : 10.56831/PSMPH-03-074
Surgical site infections [SSIs] remain a common postoperative complication despite the use of prophylactic antibiotics and other preventive measures, mainly due to increasing antimicrobial resistance. SSIs increase postoperative morbidity and mortality and may require hospital admission, intravenous antibiotics and even surgical re-intervention. A hospital based descriptive study was conducted on 100 consented postoperative patients with clinical SSIs. Data on patients was obtained using structured data collection form. Swab samples were collected aseptically from each patients. Bacteriological culture examination and identification was done following standard microbiological techniques. Antibiotics sensitivity test was done by Kirby-Bauer disc diffusion method. Ninety (90%) bacterial isolates were recovered from surgical site infection. Gram negative bacteria (GNB) were predominant (83.3%) with the dominant being Escherichia coli (27.78%) and Staphylococus aureus (16.67%). All the isolates were highly resistant to amoxicillin/clavunalate, ceftazidime, cefuroxime, levofloxacin and all the isolates were resistant to metronidazole but susceptible to imipenem, polymycin B and amikacin. The plasmid analysis in this study revealed that out of the 40 (44.4%) multi-drug resistance isolates, 35 (87.5%) of which were Gram-negative bacteria had 9 (22.5%) detectable plasmid pattern with the molecular weight of between 2027kbp to 23120kbp while the remaining 26 (74.6%) had no plasmid bands. The remaining 5 (12.3%) which was Staphylococcus aureus isolates had 2 (40%) detectable plasmid pattern with the molecular weight of between 23130kbp and 6557 while the remaining 3 (60%) had no plasmid bands. Imipenem is the drug of choice in the treatment of surgical site infections in this study area These findings necessitates judicious antibiotic use and calls for surveillance of SSIs periodically as well as strict adherence to good sanitation practice to reduce spread of drug-resistant pathogens.
Keywords: Surgical Site Infections; Antimicrobial susceptibility; Gram negative; Bacteria; Gram positive; Plasmid Profile; Clinicians; Operating Room
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